陈显军 教授 博士生导师

生物反应器工程国家重点实验室固定成员

光遗传学与合成生物学交叉学科研究中心核心成员

上海市细胞代谢光遗传学技术前沿科学研究基地成员

联系方式

电话/传真：021-64251287

E-mail: xianjunchen@ecust.edu.cn

通讯地址：上海市徐汇区梅陇路130号必赢bwin官网登录光遗传学与合成生物学交叉学科研究中心315室，邮编：200237

个人简介

2009年6月本科毕业于必赢bwin官网登录生物工程学院生物技术专业，2015年12月博士毕业于必赢bwin官网登录化学生物技术与工程专业。2016年1月开始博士后研究，先后任必赢bwin官网登录药物科学系特聘副研究员、教授。迄今已在Nature Biotechnology (2019, Cover; 2022), Nature Methods、Nature Chemical Biology (2021; 2024)、Nature Communications（2021; 2023)、Nature Protocols、Cell Research、Cell Discovery、Nucleic Acids Research (2016; 2020; 2024)、Biosensors and Bioelectronics、Journal of Controlled Release (2023; 2024)、Small等期刊发表SCI论文多篇，编写英文著作Curr Protoc Chem Biol 1个章节。已申请并授权多项中国和国际PCT发明专利。主持国家重点研发计划课题/子课题、国家自然科学基金原创/面上/青年基金、上海市自然科学基金等多项国家与省部级基金项目，担任中国生物化学与分子生物学酶学专业分委会委员兼副秘书长、上海市生物物理学会理事会理事、上海市化学化工学会生物技术专业委员会委员、九三学社上海市医疗卫生专门委员会委员，先后入选国家级高层次青年人才、中国科协青年人才托举工程、上海市青年科技启明星，获得2024世界生命科学大学“生命科学新星”、上海市生物化学与分子生物学学会第二届“青年新锐奖”、英国皇家化学会Faraday Horizon Prize（团体）、必赢bwin官网登录青年英才校长奖、必赢bwin官网登录青年五四奖章、“东岳药业”研究生奖教金特等奖。

研究方向

发展针对DNA、RNA、蛋白质等生物大分子的光遗传学监测与控制技术，解析它们在活细胞与活体内的时空动态变化规律，为探索生物大分子功能及调控机制提供实用的创新工具，同时也为活细胞与活体生物传感、即时诊断甚至实时诊断技术的发展提供新的思路。  

招生专业

生物工程专业：博士生导师/工程博士导师

药学专业：博士生导师/硕士生导师/工程硕士导师

生物与医药工程专业：工程博士导师

生物学：硕士生导师 

代表性论文（#第一作者，*通讯作者）

(1) Haifeng Zheng, Xiyu Liu, Luhui Liu, Jiarui Hu, Xianjun Chen*, Imaging of Endogenous RNA in Live Cells using Sequence-activated Fluorescent RNA probes, Nucleic Acids Research, 2024, AIP.

(2) Li Jiang^#, Fangting Zuo^#, Yuanyuan Pan^#, Ruilong Li, Yajie Shi, Xinyi Huang, Dasheng Zhang[a], Yingping Zhuang, Yuzheng Zhao, Qiuning Lin, Yi Yang, Linyong Zhu*, Xianjun Chen*, Bright and Stable Cyan Fluorescent RNA Enables Multicolour RNA Imaging in Live Escherichia coli, Small, 2024, https://doi.org/10.1002/smll.202405165.

(3) Ziyan Xu, Yongrui Liu, Fudong Li, Yi Yang, Hong Zhang, Xing Liu, Xin Xie*, Xianjun Chen*, Yunyu Shi*, Liang Zhang*, Phase separation of hnRNPA1 and TERRA regulates telomeric stability, Journal of Molecular Cell Biology, 2024, https://doi.org/10.1093/jmcb/mjae037

(4) Kaiyi Huang^#, Qianqian Song^#, Mengyue Fang^#, Deqiang Yao,Xin Shen, Xiaochen Xu, Xianjun Chen, Linyong Zhu*, Yi Yang*, Aiming Ren*, Structure-based investigation of fluorogenic Pepper aptamer, Nature Chemical Biology, 2024, https://www.nature.com/articles/s41589-021-00884-6.

(5) Fangting Zuo^#, Li Jiang^#, Ni Su^#, Yaqiang Zhang, Bingkun Bao, Limei Wang, Yajie Shi, Huimin Yang, Xinyi Huang, Ruilong Li, Qingmei Zeng, Zhengda Chen, Qiuning Lin, Yingping Zhuang, Yuzheng Zhao, Xianjun Chen*, Linyong Zhu*, Yi Yang*, Imaging the Dynamics of Messenger RNA in Live Cells with Highly Bright and Stable Green Fluorescent RNA, Nature Chemical Biology, 2024, 20, 1272–1281.

(6) Jiafeng Zou, Rui Sun, MuYe He, You Chen, Yi Cheng, Chuanhe Xia, Ying Ma, Shulei Zheng, Xiuzhi Fu, Zeting Yuan,Minbo Lan, Kaiyan Lou, Xianjun Chen*, Feng Gao*, Sequential rocket-mode bioactivating ticagrelor prodrug nanoplatform combining light-switchable diphtherin transgene system for breast cancer metastasis inhibition, ACS Appl. Mater. Interfaces, 2023, 15, 46.

(7) Renmei Liu^#, Jing Yao^#, Siyu Zhou^#, Jing Yang^#, Yaqiang Zhang, Xiaoyan Yang, Leshi Li, Yunbin Zhang, Yingping Zhuang, Yi Yang*,Xianjun Chen*, Spatiotemporal control of RNA metabolism and CRISPR-Cas functions using engineered photoswitchable RNA-binding proteins, Nature Protocols, 2024, 19, 374–405.

(8) Li Jiang^#, Xin Xie^#, Ni Su^#, Dasheng Zhang^#,Xianjun Chen^# *, Xiaochen Xu^#, Bibi Zhang, Kaiyi Huang, Jingwei Yu, Mengyue Fang, Bingkun Bao, Fangting Zuo, Lipeng Yang, Rui Zhang, Huiwen Li, Xinyi Huang, Zhengda Chen, Qingmei Zeng, Renmei Liu, Qiuning Lin, Yuzheng Zhao, Aiming Ren*, Linyong Zhu*, Yi Yang*, Large Stokes shift fluorescent RNAs for dual-emission fluorescence and bioluminescence imaging in live cells, Nature Methods, 2023, 20, 1563-1572.

(9) Yi Cheng, Jiafeng Zou, Muye He, Xinyu Hou, Hongtao Wang, Jiajun Xu, Zeting Yuan, Minbo Lan, Yi Yang, Xianjun Chen*, Feng Gao*, Spatiotemporally controlled Pseudomonas exotoxin transgene system combined with multifunctional nanoparticles for breast cancer antimetastatic therapy, Journal of Controlled Release, 2024, 367, 167-183.

(10)Dasheng Zhang^#, Zhengda Chen^#, Zengmin Du^#, Bingkun Bao^#, Ni Su^#, Xianjun Chen*, Yihui Ge, Qiuning Lin, Lipeng Yang, Yujie Hua, Shuo Wang, Xin Hua, Fangting Zuo, Ningfeng Li, Renmei Liu, Li Jiang, Chunyan Bao, Yuzheng Zhao, Joseph Loscalzo, Yi Yang* and Linyong Zhu*, Design of a palette of SNAP-tag mimics of fluorescent proteins and their use as cell reporter, Cell Discovery,2023, 9, 56.

(11) Mengyue Fang^#, Huiwen Li^#, Xin Xie^#, Hui Wang, Ying Jiang, Tianyu Li, Bibi Zhang, Xin Jiang, Yueyang Cao, Rui Zhang, Dasheng Zhang, Yuzheng Zhao, Linyong Zhu, Xianjun Chen*, Yi Yang*. Imaging intracellular metabolite and protein changes in live mammalian cells with bright fluorescent RNA-based genetically encoded sensors, Biosensors and Bioelectronics, 2023, 235, 115411.

(12) Miaowei Mao^#, Yajie Qian^#, Wenyao Zhang^#, Siyu Zhou, Zefeng Wang, Xianjun Chen*, Yi Yang*, Controlling protein stability with SULI, a highly sensitive tag for stabilization upon light induction, Nature Communications, 2023, 14, 2172.

(13) Yajie Qian^#, Ting Li^#, Siyu Zhou, Xianjun Chen*, Yi Yang*, A Single-Component Optogenetic Gal4-UAS System Allows Stringent Control of Gene Expression in Zebrafish and Drosophila, ACS Synthetic Biology, 2023, 12, 664-671.

(14) Muye He, Yuji Sun, Yi Cheng, Jie Wang, Miao Zhang, Rui Sun, Xinyu Hou, Jiajun Xu, Hai He , Hongtao Wang, Zeting Yuan, Minbo Lan, Yuzheng Zhao, Yi Yang, Xianjun Chen*, Feng Gao*, Spatiotemporally controllable diphtherin transgene system and neoantigen immunotherapy, Journal of Controlled Release, 2023, 355, 538–551.

(15) Renmei Liu^#, Jing Yang^#, Jing Yao, Zhou Zhao, Dr Wei He, Ni Su, Zeyi Zhang, Chenxia Zhang, Zhuo Zhang, Haibo Cai, Linyong Zhu, Yuzheng Zhao, Shu Quan, Xianjun Chen*, Yi Yang*, Optogenetic control of RNA function and metabolism using engineered light-switchable RNA-binding proteins, Nature Biotechnology, 2022, 40, 779-786.

(16) Kaiyi Huang^#, Xianjun Chen^#, Chunyan Li, Qianqian Song, Huiwen Li, Linyong Zhu*, Yi Yang*, Aiming Ren*, Structure-based investigation of flu0orogenic Pepper aptamer, Nature Chemical Biology, 2021, 17, 1289-1295.

(17) Ting Li^#, Xianjun Chen^#, Yajie Qian, Jiawei Shao, Xie Li, Shuning Liu, Linyong Zhu, Yuzheng Zhao, Haifeng Ye* & Yi Yang*, A synthetic BRET-based optogenetic device for pulsatile transgene expression enabling glucose homeostasis in mice, Nature Communications, 2021, 12, 615.

(18) Xinyu Hou, Chenting Shou, Muye He, Jiajun Xu, Yi Cheng, Zeting Yuan, Minbo Lan, Yuzheng Zhao, Yi Yang, Xianjun Chen*, Feng Gao*, A combination of LightOn gene expression system and tumor microenvironment-responsive nanoparticle delivery system for targeted breast cancer therapy, Acta Pharmaceutica Sinica B, 2020, 10, 1741-1753.

(19) Xie Li, Changcheng Zhang, Xiaopei Xu, Jun Miao, Jing Yao, Renmei Liu, Yuzheng Zhao, Xianjun Chen*, Yi Yang*. A single-component light sensor system allows highly tunable and direct activation of gene expression in bacterial cells, Nucleic Acids Research, 2020, 48, e33.

(20) Jiajun Xu, Muye He, Xinyu Hou,Yan Wang,Chenting Shou, Xiaoran Cai, Zeting Yuan, Yu Yin, Minbo Lan, Kaiyan Lou, Yuzheng Zhao, Yi Yang, Xianjun Chen*, Feng Gao*, Safe and Efficacious Diphtheria Toxin-Based Treatment for Melanoma: Combination of a Light-On Gene-Expression System and Nanotechnology, Molecular pharmaceutics, 2020, 17 (1), 301-315.

(21) Xianjun Chen^#, Dasheng Zhang^#, Ni Su^#, Bingkun Bao, Xin Xie, Fangting Zuo, Lipeng Yang, Hui Wang, Li Jiang, Qiuning Lin, Mengyue Fang, Ningfeng Li, Xin Hua, Zhengda Chen, Chunyan Bao, Jinjin Xu, Wenli Du, Lixin Zhang, Yuzheng Zhao, Linyong Zhu*, Joseph Loscalzo, Yi Yang*, Visualizing RNA Dynamics in Live Cells with Bright and Stable Fluorescent RNAs, Nature Biotechnology, 2019, 37, 1287-1293.

(22) Xiaopei Xu, Zhaoxia Du, Renmei Liu, Ting Li, Yuzheng Zhao, Xianjun Chen* and Yi Yang*, A Single-Component Optogenetic System Allows Stringent Switch of Gene Expression in Yeast Cells, ACS Synthetic Biology, 2018, 7, 2045-2053.

(23) Wansheng Sun, Wenyao Zhang, Chao Zhang, Miuwei Mao, Yuzheng Zhao, Xianjun Chen*, and Yi Yang*, Light-induced protein degradation in human-derived cells, Biochemical and Biophysical Research Communications, 2017, 487, 241-246.

(24) Xianjun Chen, Renmei Liu, Zhengcai Ma, Xiaopei Xu., Haoqian Zhang, Jianhe Xu, Qi Ouyang, and Yi Yang*, An extraordinary stringent and sensitive light-switchable gene expression system for bacterial cells, Cell Research, 2016, 26,  854-857.

(25) Xianjun Chen^#, Ting Li^#, Xue Wang, Zengmin Du, Renmei Liu, and Yi Yang*, Synthetic dual-input mammalian genetic circuits enable tunable and stringent transcription control by chemical and light, Nucleic Acids Research, 2016, 44, 2677-2690.

(26) Xianjun Chen, Ting Li, Xue Wang, and Yi Yang*, A light-switchable bidirectional expression module allowing simultaneous regulation of multiple genes, Biochemical and Biophysical Research Communications, 2015, 465, 769-776.

(27) Xue Wang, Xianjun Chen, and Yi Yang*, Spatiotemporal control of gene expression by a light-switchable transgene system, Nature Methods, 2012, 9, 266-269.